Water content of culture material: If the water content of the medium is too high, the ventilation will be affected, hyphae will grow slowly due to lack of oxygen, moisture will be deposited in the lower half of the cultivation bag, and the hypha length will not be able to go. Please refer to the following standards: 1. Grain food: ±42% moisture 2. Mushroom culture material: ±62% moisture Please handle it carefully. Read more Sealing method：Our these mushroom bags can be sealed with an impulse sealer, or just tie to seal . these bags are durable and can be reusable in most times. Sterilization Continuous sterilization at a sufficiently high temperature, high-temperature autoclave sterilization, sterilization speed is 6-7 times normal temperature, steam can quickly penetrate the bacteria bag, to achieve sterilization effect, bacteria bag should be placed in a "hollow type", in the middle Forms a square hollow with 2 to 4 pockets space, so that the penetration is stronger, the temperature rises faster, and there is no dead angle in all directions. Read more After the high-temperature steam temperature was maintained at 125°C for 10 minutes until the center of the most concentrated bag culture material, the temperature was maintained at 125 degrees for about 150 minutes, and the sterilization was basically completed. cool down In a dry, well-ventilated space, open the folded bag mouth while ensuring the supply of sterile compressed air. Vaccination Sterilize all utensils before inoculation and complete in aseptic room sterile conditions. seal Under the same aseptic conditions, the carbon dioxide content of the ambient air is completed up to 2500 PPM by double-sided heat welding, thus ensuring dry ventilation. Preserved bacteria It needs to be stored in a cool, dry environment.